![]() These hub RBPs may provide potential biomarkers for diagnosis and prognosis. ![]() Moreover, by unveiling a number of tongue cancer prognosis-related hub RBPs, our results may shed new light on understanding RBPs-directed network underlying tongue cancer progression. GO and KEGG analyses were carried out to reveal the underlying functional mechanisms in metabolic processes of RBPs in tongue cancer. By utilizing differential expression analysis and WGCNA, we constructed the link between the expression and tongue cancer patients' clinical features. In the present study, we downloaded the RNA-seq and clinical datasets from TCGA and GEO. Thus, global analysis of the function impact of RBPs will provide better understanding of tongue cancer and new insight into tongue cancer therapy. However, it remains largely unclear of the role of RBPs in tongue cancer. Several investigations have shown that RBPs are dysregulated in different cancer types, and can impact on the expression and function of oncoproteins and tumor-suppressor proteins. These RBPs establish highly dynamic interactions with other proteins, coding and/or noncoding RNAs, and influence a variety of physiological and pathological processes including cancer. To date, more than 3800 human RBPs were determined in diverse cell types. RNA binding proteins (RBPs) are a class of proteins that interacting with a variety type of RNAs. Hence, expanding our understanding of the pathophysiological mechanism to develop effective methods for early screening and diagnosis and identification of new therapeutic targets are urgently required for tongue cancer. Thus, even the currently understanding of cancer development and progression is rapidly increasing, tongue cancer is still a serious health issue in many counties with significantly lower 5-year survival rate in patients. This feature causes delay in diagnosis and thus leading to poor prognosis. This is partly due to that tongue cancer is comparatively silent and progresses fast from a premalignant state into invasive carcinoma. Tongue cancer is the most frequent type of malignancy in the oral cavity, and is characterized by remarkable aggressive biological behavior. Keywords: RNA binding protein, tongue cancer, metabolic process, risk score, prognostic model. Together, our study revealed the underlying role of RBP in tongue cancer biology and potentially unveiled novel targets for cancer therapy. As suggested in functional annotation analysis, we found an intensive enrichment of these prhRBPs in metabolic pathways, including AMPK, HIF-1 signaling pathway, Glycolysis, and steroid hormone biosynthesis. The time-dependent receiver operating characteristic (ROC) analysis of the prognostic model further confirmed the predictive capability of the risk model for tongue cancer. In addition, we validated the prognostic model in GSE41613, another tongue cancer patient cohort from GEO datasets. Based on these hub RBPs, we constructed a prognostic model and found that the patients in the high-risk group had dramatically poor overall survival compared to those in low-risk group. Among them, six RBPs (PGK1, SLC20A1, LEPR, CYP19A1, ZC3H12D, and PFKM) were shown to be the prognosis-related hub RBPs (prhRBPs). Furthermore, we investigated the expression and prognostic value of these RBPs and their highly correlated networks. Then, in a protein-protein interaction (PPI) network containing 134 nodes (RBPs) and 315 network edges (RBP-RBP interacting networks), the top 30 hub RBPs were identified using the CytoHubba plugin of Cytoscape. Here, by integrating the differential gene expression analysis and the Weighted Gene Co-expression Network Analysis (WGCNA) of TCGA-retrieved RNA-seq data, we identified a total of 171 differential co-expression RBPs. ![]() ![]() However, the role of RBPs in tongue cancer are largely unclear. RNA binding proteins (RBPs) are dysregulated and associated with the occurrence and development in various malignant tumors. Select the file that you have just downloaded and select import option Reference Manager (RIS). Identification of Prognosis-related Hub RNA Binding Proteins Function through Regulating Metabolic Processes in Tongue Cancer.
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